LIPA Knockout cell line (SH-SY5Y)

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LIPA Knockout cell line (SH-SY5Y) Order

LIPA Knockout cell line (SH-SY5Y)
Catalog#
YKO-H261
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LIPA Knockout cell line (SH-SY5Y)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LIPA knockout cell line (SH-SY5Y)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
LIPA
Gene ID
3988
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_E1C5(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript LIPA-202)
Validation: PCR+Sanger Sequencing
LIPA Knockout cell line (SH-SY5Y) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SH-SY5Y		 STR Info (Cell bank)
Cell Line:SH-SY5Y
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11111111
D7S820 710710
D16S539 813813
VWA 14181418
TH01 710710
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 1822
FGA 23.224
D2S1338 1719
D21S11 3131.2
D18S51 1316
D8S1179 1515
D3S1358 1516
D6S1043 1218
PENTAE 711
D19S433 1314
PENTAD 1012
D1S1656 1212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LIPA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SH-SY5Y cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LIPA gene knockout LIPA knockout cell line (SH-SY5Y) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LIPA Knockout cell line (SH-SY5Y)
LIPA Knockout cell line (SH-SY5Y)
Catalog#
YKO-H261
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • LIPA Knockout cell line (Hep G2)

    LIPA Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT3075
    YKO-HT3075(Hep G2)
  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
    YKO-HT0652(THP-1)
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
    YKO-XN90098(HeLa)

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    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • LIPA Knockout cell line (Hep G2)

    LIPA Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT3075
      inquiry   More details
  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
      inquiry   More details
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
      inquiry   More details

LIPA Knockout cell line (SH-SY5Y) Order

LIPA Knockout cell line (SH-SY5Y)
Catalog#
YKO-H261
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LIPA Knockout cell line (SH-SY5Y)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LIPA knockout cell line (SH-SY5Y)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
LIPA
Gene ID
3988
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_E1C5(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript LIPA-202)
Validation: PCR+Sanger Sequencing
LIPA Knockout cell line (SH-SY5Y) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SH-SY5Y		 STR Info (Cell bank)
Cell Line:SH-SY5Y
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11111111
D7S820 710710
D16S539 813813
VWA 14181418
TH01 710710
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 1822
FGA 23.224
D2S1338 1719
D21S11 3131.2
D18S51 1316
D8S1179 1515
D3S1358 1516
D6S1043 1218
PENTAE 711
D19S433 1314
PENTAD 1012
D1S1656 1212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LIPA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SH-SY5Y cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LIPA gene knockout LIPA knockout cell line (SH-SY5Y) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LIPA Knockout cell line (SH-SY5Y)
LIPA Knockout cell line (SH-SY5Y)
Catalog#
YKO-H261
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • LIPA Knockout cell line (Hep G2)

    LIPA Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT3075
    YKO-HT3075(Hep G2)
  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
    YKO-HT0652(THP-1)
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
    YKO-XN90098(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • LIPA Knockout cell line (Hep G2)

    LIPA Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT3075
      inquiry   More details
  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
      inquiry   More details
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
      inquiry   More details

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